Wide-field three-photon excitation in biological samples
نویسندگان
چکیده
منابع مشابه
Wide-field three-photon excitation in biological samples
Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photo...
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Multiphoton microscopy is becoming a popular mode of live and fixed cell imaging. This mode of imaging offers several advantages due to the fact that fluorochrome excitation is a nonlinear event resulting in excitation only at the plane of focus. Multiphoton excitation is enhanced by the use of ultrafast lasers emitting in the near IR, offering better depth penetration coupled with efficient ex...
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ژورنال
عنوان ژورنال: Light: Science & Applications
سال: 2016
ISSN: 2047-7538
DOI: 10.1038/lsa.2016.255